P5459
Protéase from Bacillus licheniformis
glycerol solution (50%)
Synonyme(s) :
Protéinase from Bacillus licheniformis, Subtilisine A
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Numéro CE :
MDL number:
Specific activity:
≥7 units/mg protein (biuret)
Biological source:
Bacillus licheniformis
Concentration:
≥50 mg/mL
Service technique
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Laissez-nous vous aiderbiological source
Bacillus licheniformis
Quality Segment
form
glycerol solution (50%)
specific activity
≥7 units/mg protein (biuret)
concentration
≥50 mg/mL
solubility
0.85% sodium chloride: soluble, clear (Diluted), H2O: miscible
storage temp.
2-8°C
General description
The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.
Application
Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.
Packaging
Package size based on protein content.
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
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Mot-clé
Danger
Codes de danger
Mentions de précaution
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
Classe de stockage
10 - Combustible liquids
Que se passe-t-il ?
WGK 2
EPI (équipement de protection individuelle)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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