P2645
Protein Kinase A Catalytic Subunit from bovine heart
≥9 units/μg protein (cyclic-AMP is not required for this activity), lyophilized (white powder to sticky mass to hard pellet)
Synonyme(s) :
PKA
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About This Item
Numéro MDL:
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.32
Produits recommandés
Forme
lyophilized (white powder to sticky mass to hard pellet)
Niveau de qualité
Activité spécifique
≥9 units/μg protein (cyclic-AMP is not required for this activity)
Poids mol.
40,862 Da
Température de stockage
−20°C
Description générale
Protein Kinase A enzyme is composed of two subunits- catalytic and regulatory. The catalytic subunit exists as a monomer in the presence of cAMP and has a molecular weight of 40,862 Da.
Application
Protein Kinase A (PKA) Catalytic Subunit from bovine heart has been used-
- to study PKA-mediated inhibition of IRK1 (inwardly rectifying K+) channels
- in in Vitro PKA pPhosphorylation assay
- in Vitro affinity binding assays
- to study effects of PKA on inspiratory drive currents in functionally active motorneurons
Actions biochimiques/physiologiques
Protein Kinase A (PKA) controls the transduction of Hedgehog signaling and participates in proliferation and fate specification. It phosphorylates several neurotransmitter receptors, transcription factors and constituents of various intracellular signaling pathways.
Protein Kinase A catalyzes the transfer of terminal phosphate from ATP to threonine or serine residues present on various proteins. This protein is inactive in the absence of cAMP, where the catalytic and regulatory subunits are bound together. The regulatory subunit, in the presence of cAMP, binds to cAMP and releases the catalytic subunit.
Conditionnement
Package size based on phosphorylating units
Définition de l'unité
Phosphorylating Activity: One unit will transfer 1.0 picomole phosphate from ATP to hydrolyzed and partially dephosphorylated casein per minute at pH 6.5 at 30°C, determined by measuring the production of ADP.
Forme physique
Lyophilized powder with sucrose and phosphate buffer salts as stabilizer.
Notes préparatoires
Prepared from protein kinase A (P 5511)
Clause de non-responsabilité
Please note that the pack size has been changed to align with the unit definition, while the number of phosphorylating units remain the same as before.
Inhibiteur
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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