MAK063

L-Carnitine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonyme(s) : Carnitine Quantification Kit
• NACRES: NA.84
• UNSPSC Code: 12161503

Propriétés

• usage: sufficient for 100 colorimetric or fluorometric tests
• detection method: colorimetric, fluorometric
• storage temp.: −20°C

Description

General description

Carnitine is a metabolite that is synthesized from lysine and methionine. Carnitine is essential for fatty acid β-oxidation due to its role in fatty acid transport into the mitochondrial matrix via the carnitine/acylcarnitine shuttle. Carnitine exists in two stereoisomers but only the L-carnitine isomer is biologically active. Tissue levels of L-carnitine decrease with aging and decreased L-carnitine may contribute to age-related mitochondrial decline. The L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum.

Application

Suitable for the measurement of free L-Carnitine in biological samples such as tissue, cells and serum

L-Carnitine Assay Kit has been used to determine the concentration of carnitine in serum samples.

Biochem/physiol Actions

L-carnitine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the L-carnitine present. Typical sensitivities of detection for this kit are 10 μM (colorimetric) and 1 μM (fluorometric). The normal range for serum L-Carnitine is between 10-70 μM.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mot-clé: Danger
• Codes de danger: H315,H319,H334,H335
• Mentions de précaution: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): 188.6 °F - closed cup
• Point d'éclair (°C): 87 °C - closed cup
• Que se passe-t-il ?: WGK 3