M0644

Milieu essentiel minimum d′Eagle

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

• Synonyme(s) : MEM
• NACRES: NA.75
• UNSPSC Code: 12352207

Propriétés

• Quality Segment: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• components: NaHCO₃: no; L-glutamine: yes; phenol red: yes; HEPES: no; sodium pyruvate: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Application

Minimum essential medium Eagle has been used to:

• maintain natural killer (NK) cell lines
• culture Leishmania donovani strains
• culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles

Preparation Note

Formulated to contain 10.1 grams of powder per liter of medium.

Supplement with 2.2 g/L sodium bicarbonate.

Informations sur la sécurité

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable