L6274

Laminin from human placenta

liquid, BioReagent, suitable for cell culture

• Synonyme(s) : Cell culture grade laminin, Human Laminin
• Numéro CAS: 114956-81-9
• Numéro MDL: MFCD00081739
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: human placenta
• Niveau de qualité: 300
• Gamme de produits: BioReagent
• Forme: liquid
• Concentration: 0.5 mg/mL in Tris-buffered saline
• Technique(s): cell culture | mammalian: suitable
• Couverture de surface: 1‑2 μg/cm²
• Numéro d'accès UniProt: P07942
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... LAMB1(3912)

Description

Application

Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm². The optimal concentration does depend on cell type as well as the application or research objectives.

Actions biochimiques/physiologiques

Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.

Composants

Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.

Attention

It is recommended to store this product at -70°C, where it will remain stable for two years.

Notes préparatoires

This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable