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L5158

Sigma-Aldrich

Leukemia Inhibitory Factor from mouse

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Synonyme(s) :

LIF

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About This Item

Numéro MDL:
MFCD00283681
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Leukemia Inhibitory Factor from mouse, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture

Produit recombinant

expressed in E. coli

Niveau de qualité

200

Pureté

≥95% (SDS-PAGE)

Forme

buffered aqueous solution

Qualité

endotoxin tested

Technique(s)

cell culture | mammalian: suitable

Température de stockage

2-8°C

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Application

Leukemia Inhibitory Factor from mouse has been used:
  • as a component of enriched co-culture media
  • as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells
  • as a component in maintenance medium for maintaining embryonic stem cells
  • to treat ovaries in ovarian culture

Actions biochimiques/physiologiques

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Forme physique

Solution in phosphate buffered saline containing 0.02% TWEEN® 20

Remarque sur l'analyse

The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.

Informations légales

TWEEN is a registered trademark of Croda International PLC

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

C-Kit Positive Cardiac Stem Cells and Bone Marrow-Derived Mesenchymal Stem Cells Synergistically Enhance Angiogenesis and Improve Cardiac Function After Myocardial Infarction in a Paracrine Manner
Bao L, et al.
Journal of Cardiac Failure, 23(5), 403-415 (2017)
Renée F de Pooter et al.
Methods in molecular biology (Clifton, N.J.), 330, 113-121 (2006-07-19)
Mature hematopoietic cells, like all other terminally differentiated lineages, arise during ontogeny via a series of increasingly restricted intermediates. Hematopoietic progenitors have their origin in the mesoderm, which gives rise to hemangioblasts that can differentiate into endothelial or endocardial precursors
LIF-immobilized nonwoven polyester fabrics for cultivation of murine embryonic stem cells
Cetinkaya G, et al.
Journal of Biomedical Materials Research Part A, 81(4), 911-919 (2007)
Cristian Soitu et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(23), 2001854-2001854 (2020-12-12)
There is an unmet demand for microfluidics in biomedicine. This paper describes contactless fabrication of microfluidic circuits on standard Petri dishes using just a dispensing needle, syringe pump, three-way traverse, cell-culture media, and an immiscible fluorocarbon (FC40). A submerged microjet
A Rodríguez et al.
Theriogenology, 67(5), 1092-1095 (2007-01-11)
Leukemia inhibitory factor (LIF) is a cytokine that shows conflicting effects on in vitro produced (IVP) bovine embryos. Bovine LIF (bLIF) has been cloned and used in culture, but there is no commercially available bLIF. Thus, researchers use human LIF
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Articles

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

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