H4149
Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse
clone LK1, ascites fluid
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About This Item
Produits recommandés
Source biologique
mouse
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
LK1, monoclonal
Poids mol.
antigen 60 kDa
Contient
15 mM sodium azide
Espèces réactives
rat, human, chicken
Technique(s)
electron microscopy: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 1:400 using cultured human foreskin fibroblast extract
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... HSPD1(3329)
rat ... Hspd1(63868)
Description générale
Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.
Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.
Spécificité
The antibody recognizes an epitope located between amino acid residues 383-447 of the human HSP 60. It is not cross-reactive with the bacterial counterpart (E. coli), or with helminth and spinach HSP60. The antibody reacts with constitutive and inducible HSP60. It demonstrates a raised level of staining in immunohistochemistry of synovial membranes taken from patients with juvenile chronic arthritis.
Immunogène
recombinant human heat shock protein 60 (HSP60)
Application
Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:
- Enzyme linked immunosorbent assay (ELISA)
- Immunoblotting
- Immunocytochemistry
- Immunohistochemistry
- Immunoelectron microscopy
- Double immunofluorescence
Actions biochimiques/physiologiques
A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.
Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.
Forme physique
Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Antonella Marino Gammazza et al.
Cell stress & chaperones, 19(3), 343-353 (2013-09-24)
The role Hsp60 might play in various inflammatory and autoimmune diseases is under investigation, but little information exists pertaining to Hashimoto's thyroiditis (HT). With the aim to fill this gap, in the present work, we directed our attention to Hsp60
Studies characterizing 60 kda autoantibodies in subjects with schizophrenia
Wang X, et al.
Biological Psychiatry, 53(5), 361-375 (2003)
HSP60: issues and insights on its therapeutic use as an immunoregulatory agent
Coelho V and Faria A
Frontiers in Immunology, 2, 97-97 (2012)
Francesco Cappello et al.
Cancer, 107(10), 2417-2424 (2006-10-19)
The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequency of adenocarcinoma has been reported in patients with COPD. Heat shock proteins (Hsps) are implicated in tumoral cell
P Costantini et al.
Cell death and differentiation, 9(1), 82-88 (2002-01-23)
As shown here, mitochondria purified from different organs (liver, brain, kidney, spleen and heart) contain both pro-caspase-9 and the processed, mature form of caspase-9. Purified liver mitochondria release mature caspase-9 upon induction of permeability transition in vitro. This is accompanied
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