H0654

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H₂O

• Synonyme(s) : Hygromycin B, Hygrovetine
• Formule empirique (notation de Hill) : C₂₀H₃₇N₃O₁₃
• Numéro CAS: 31282-04-9
• Poids moléculaire : 527.52
• UNSPSC Code: 51102829
• NACRES: NA.85
• PubChem Substance ID: 24895410
• MDL number: MFCD06795479
• Beilstein/REAXYS Number: 6755837

Propriétés

• biological source: Streptomyces hygroscopicus
• Quality Segment: 200
• sterility: 0.22 μm filtered
• concentration: ≥60% (HPLC), 45-60 mg/mL in H₂O
• color: brown-yellow, light orange to dark orange, light yellow to dark yellow
• antibiotic activity spectrum: viruses
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
• InChI key: GRRNUXAQVGOGFE-XKIAHZFYSA-N

Description

General description

Chemical structure: aminoglycoside

Application

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Biochem/physiol Actions

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Preparation Note

This product is a solution that comes =60% (HPAE) and 45-60 mg/mL in H₂O.

Disclaimer

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Informations sur la sécurité

• pictograms: GHS06
• signalword: Danger
• hcodes: H301 + H311,H330
• pcodes: P260 - P264 - P270 - P280 - P302 + P352 + P312 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral
• Classe de stockage: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 3
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter