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F7053

Sigma-Aldrich

Anticorps monoclonal anti-vinculine antibody produced in mouse

clone hVIN-1, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Vinculin antibody produced in mouse

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About This Item

Numéro MDL:
MFCD00162905
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

FITC conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

hVIN-1, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 116 kDa

Espèces réactives

frog, chicken, mouse, canine, human, bovine, rat, turkey

Conditions de stockage

protect from light

Technique(s)

direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells

Isotype

IgG1

Numéro d'accès UniProt

P18206

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

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Spécificité

Marquage spécifique de la vinculine au niveau des contacts cellule/cellule et cellule/substrat. Forte réactivité avec la vinculine humaine. Réactivité croisée avec la métavinculine de muscle lisse.

Immunogène

Vinculine d'utérus humain purifiée.

Application

Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.

Actions biochimiques/physiologiques

Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Mathieu Maalouf et al.
Nanomaterials (Basel, Switzerland), 12(10) (2022-05-29)
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Akiko Hashimoto-Tane et al.
The Journal of experimental medicine, 213(8), 1609-1625 (2016-06-30)
The immunological synapse (IS) formed at the interface between T cells and antigen-presenting cells represents a hallmark of initiation of acquired immunity. T cell activation is initiated at T cell receptor (TCR) microclusters (MCs), in which TCRs and signaling molecules
Viorel-Iulian Suica et al.
Proteome science, 13, 30-30 (2015-12-03)
Membrane microdomains represent dynamic membrane nano-assemblies enriched in signaling molecules suggesting their active involvement in not only physiological but also pathological molecular processes. The hyperlipidemic stress is a major risk factor of atherosclerosis, but its exact mechanisms of action at
Subhashree Shivani et al.
Frontiers in cell and developmental biology, 10, 825791-825791 (2022-04-09)
The mediation of the extracellular matrix is one of the major environmental cues to direct cell migration, such as stiffness-dependent durotaxis and adhesiveness-dependent haptotaxis. In this study, we explore another possible contact guidance: roughness dependent topotaxis. Different from previously reported
Carol Jurchenko et al.
Biophysical journal, 106(7), 1436-1446 (2014-04-08)
The interplay between chemical and mechanical signals plays an important role in cell biology, and integrin receptors are the primary molecules involved in sensing and transducing external mechanical cues. We used integrin-specific probes in molecular tension fluorescence microscopy to investigate
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