F4021

Follicle Stimulating Hormone from human pituitary

~7,000 IU/mg (powder)

• Synonyme(s) : FSH, Follitropin
• Numéro CAS: 9002-68-0
• UNSPSC Code: 51111800
• eCl@ss: 42020504
• NACRES: NA.32
• MDL number: MFCD00131099
• Biological source: human pituitary glands
• Form: powder

Propriétés

• biological source: human pituitary glands
• Quality Segment: 200
• sterility: non-sterile
• form: powder
• specific activity: ~7,000 IU/mg (powder)
• mol wt: 35 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: HBsAg, HCV and HIV-1/HIV-2, tested negative
• pH: 7.4
• solubility: water: ~50 g/L
• UniProt accession no.: P01225, P23945
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... FSHB(2488), FSHR(2492)

Description

General description

The α chain of FSH has 89 amino acids and the β chain has 115 amino acids. The α chain is not active. FSH belongs to glycoprotein hormone family. It is secreted by the gonadotropic cells of pituitary gland.
Research area: Cell signaling

Application

Follicle stimulating hormone (FSH) from human pituitary has been used for-

• in vitro luteinization of Macaque granulosa cells
• luteal cell proliferation assay
• culturing of preantral follicle cells obtained from mice
• in vitro maturation of immature cumulus-oocyte complexes (COCs) obtained from mice and
• culture of RAW264.7 cell line

Biochem/physiol Actions

FSH (follicle stimulating hormone) in involved in the growth of ovarian follicles and steroidogenesis of ovaries. FSHR (follicle-stimulating hormone receptor) interacts with FSH in the ovaries and determines the responsiveness to this hormone. Depressed levels of FSHR result in poor responsiveness and influence folliculogenesis. In the Caucasian population, certain polymorphisms in the FSHR gene are more commonly found in patients with polycystic ovary syndrome (PCOS). In the male fetuses and neonates, it facilitates the proliferation of Sertoli cells. In pubescent males, it stimulates spermatogonia to undergo mitosis.FSH upon binding to its receptor FSH-R positively regulates G-protein coupled receptors/cyclic adenosine monophosphate (cAMP)/protein kinase A pathways to cascade intracellular signals.

The α-chain is not active; biological specificity is attributed to the β-chain. Induces maturation of Graafian follicles of the ovary; promotes development of germinal cells in males; activates cytosolic tyrosine kinase.

Physical form

Contains 0.1 mg of 0.05M PBS buffer (pH 7.4)

Other Notes

Two-chain glycoprotein hormone

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable