E3789

Amphotericin B – Low Endotoxin

Less than 5 EU/mg, suitable for cell culture

• Synonyme(s) : Amphotericin B – Low Endotoxin
• NACRES: NA.76
• UNSPSC Code: 12352207

Propriétés

• biological source: Streptomyces sp. (Microbial)
• form: powder
• mol wt: 924.08
• technique(s): cell culture | mammalian: suitable
• storage temp.: 2-8°C

Description

General description

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols,primarily ergosterols, of fungal and bacterial cell membranes.After binding to sterols, it forms channels in the membranes, causing small molecules to leak out.Amphotericin B is effective against fungi and yeast.The name of the drug is derived from the amphoteric behavior of the drug, due to the carboxyl group on the main ring and a primary amino group on the mycosamine ring.
Amphotericin B induces K+ leakage, which is separate from its lethal action, as was demonstrated in human erythrocytes and is due to the inhibitory effect on the Na+/K+ pump.At sub-lethal concentrations, this drug stimulates either the activity of some membrane enzymes or cellular metabolism,in particular stimulation of some cells of the immune system.

Preparation Note

Amphotericin B remains active for 3 days in culture at 37°C. For long term, storage at 2−8°C, protected from air and light, is recommended. Under these conditions the products remain active for 5 years.

If reconstituted at 25 mg/10 mL of sterile water, there is no need to filter sterilize. This will yield a slightly hazy yellow solution.

Informations sur la sécurité

• pictograms: GHS08
• signalword: Danger
• hcodes: H372
• pcodes: P260 - P264 - P270 - P314 - P501
• Hazard Classifications: STOT RE 1
• Classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable