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D4154

Plasmid DNA from E. coli RRI

pUC18, buffered aqueous solution

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A propos de cet article

Numéro CAS:
9007-49-2
UNSPSC Code:
41105600
NACRES:
NA.85
MDL number:
MFCD00166896
eCl@ss:
32160414
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biological source

Escherichia coli (RRI)

Quality Segment

200

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

General description

Plasmid DNA from Escherichia coli RRI confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene. pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.

Application

Plasmid DNA from E. coli RRI has also been used for in vitro DNA transfection studies.
Plasmid DNA from Escherichia coli RRI has been used in polymerase chain reaction (PCR).

Biochem/physiol Actions

Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.
These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.

Other Notes

pUC18 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

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D3404D3035262012
Plasmid DNA from E. coli RRI pUC18, buffered aqueous solution

Sigma-Aldrich

D4154

Plasmid DNA from E. coli RRI

Plasmid DNA from E. coli RRI pUC19, buffered aqueous solution

Sigma-Aldrich

D3404

Plasmid DNA from E. coli RRI

Deoxyribonucleic acid from human placenta buffered aqueous solution, sexed, female

Sigma-Aldrich

D3035

Deoxyribonucleic acid from human placenta

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes Purified DNA from salmon testes. Absorption (0.001% solution in phosphate buffer, pH 7.0, 260 nm): ≥0.155.

Millipore

262012

Deoxyribonucleic Acid, Sodium Salt, Salmon Testes

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

biological source

Escherichia coli (RRI)

biological source

-

biological source

human placenta

biological source

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–2 of 2 Questions  

  1. What is the sequence of plasmid pUC18 (product number D4154) and the number of copies of the plasmid contained in this product? It will be used as an internal control for PCR amplification.

    What is the sequence of plasmid pUC18 (product number D4154) and the number of copies of the plasmid contained in this product? It will be used as an internal control for PCR amplification.

    1 answer

    1. The GenBank accession number for the sequence of the high-copy number plasmid pUC18 is VB0025.

      The following link contains the sequence for this plasmid: https://www.addgene.org/50004/sequences/#depositor-full.

      The plasmid is 2,686 base pairs in length and the molecular weight is approximately 1.75 × 10⁶ daltons, so the 10µg should be 3.4 x 10^12 copies.

      Helpful?

  2. user manual for Puc18?

    1 answer

    1. The Puc18 is not supplied with a user manual or specific protocol. Please see the link below to the product data sheet which provides additional information on this plasmid: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/263/858/d4154dat.pdf

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