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D4138

Deoxyribonuclease II from porcine spleen

Name: Deoxyribonuclease II from porcine spleen
Brand: SIGMA

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonyme(s) :

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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A propos de cet article

Numéro CAS:

9025-64-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-801-8

MDL number:

MFCD00130919

Numéro CE :

3.1.22.1 (BRENDA, IUBMB)

Specific activity:

2,000-6,000 Kunitz units/mg protein (biuret)

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Propriétés

type

Type IV

Quality Segment

200

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

input

general use sample(s)

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Description

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Physical form

Contains sodium chloride

Other Notes

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg²⁺] = 0.83 mM

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Cet article	D8764	D4513	D4527
Sigma-Aldrich D4138 Deoxyribonuclease II from porcine spleen	Sigma-Aldrich D8764 Deoxyribonuclease II from bovine spleen	Sigma-Aldrich D4513 Désoxyribonucléase I from bovine pancreas	Sigma-Aldrich D4527 Désoxyribonucléase I from bovine pancreas
description Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)	description Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein	description Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein	description Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein
specific activity 2,000-6,000 Kunitz units/mg protein (biuret)	specific activity ≥1,000 units/mg protein	specific activity ≥2,000 units/mg protein	specific activity ≥2,000 units/mg protein
form lyophilized powder	form essentially salt-free, lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 45 kDa	mol wt 38 kDa	mol wt ~31 kDa	mol wt ~31 kDa
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 300
foreign activity RNase ≤0.05%	foreign activity RNase ≤0.1%	foreign activity Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%	foreign activity Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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