D1529

1-Fluoro-2,4-dinitrobenzene

≥99%

• Synonyme(s) : 2,4-Dinitro-1-fluorobenzene, DNFB, DNPF, FDNB, Sanger reagent
• Formule linéaire : (O₂N)₂C₆H₃F
• Numéro CAS: 70-34-8
• Poids moléculaire : 186.10
• Numéro Beilstein : 398632
• Numéro CE : 200-734-3
• Numéro MDL: MFCD00007056
• Code UNSPSC : 12352204
• ID de substance PubChem : 24893470
• Nomenclature NACRES : NA.83

Propriétés

• Pureté: ≥99%
• Forme: solid
• Indice de réfraction: n20/D 1.569 (lit.)
• Point d'ébullition: 178 °C/25 mmHg (lit.)
• Pf: 25-27 °C (lit.)
• Densité: 1.482 g/mL at 25 °C (lit.)
• Température de stockage: room temp
• Chaîne SMILES: [O-][N+](=O)c1ccc(F)c(c1)[N+]([O-])=O
• InChI: 1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H
• Clé InChI: LOTKRQAVGJMPNV-UHFFFAOYSA-N

Description

Description générale

1-Fluoro-2,4-dinitrobenzene (DNFB) is a reagent that modifies the N-terminal amino acids of proteins and peptides. DNFB is also commonly known as Sanger′s Reagent, named after Frederick Sanger, who reported in a classic 1945 paper the use of DNFB for derivatization of proteins and peptides specifically at their N-termini.†

Application

1-Fluoro-2,4-dinitrobenzene has been used to induce severe contact hypersensitivity in mice and to monitor gene expression by in vivo imaging method, and to induce chronic itch as a model for analysing itch-scratching behavior.

Actions biochimiques/physiologiques

1-Fluoro-2,4-dinitrobenzene is a contact-sensitizing hapten, commonly used in experimental studies on contact hypersensitivity, inducing itch model, and atopic dermatitis. Mast cell activation is a key step in the DNFB induced sensitivity. DNFB covalently binds to the N-terminal amino acid of the protein and aids in Sanger sequencing.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302,H315,H317,H319,H335,H373
• Conseils de prudence: P260 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P314
• Classification des risques: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 327.2 °F
• Point d'éclair (°C): 164 °C
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves