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CASP3C

Sigma-Aldrich

Caspase 3 Assay Kit, Colorimetric

Synonyme(s) :

Colorimetric Caspase-3 Activity Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
 sufficient for 100 standard tests (1 ml)

Niveau de qualité

300

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

human ... CASP3(836)

Description générale

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Application

The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.

Définition de l'unité

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Autres remarques

Use 17 megohm water only

Composants de kit seuls

Réf. du produit
Description
  • Water (17-18 megaOhm) 125 mL

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgFDS
  • A2559Ac-DEVD-pNA substrate 15 mgFDS
  • p-Nitroaniline standard 1 mg

Pictogrammes

Skull and crossbonesHealth hazard
GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Point d'éclair (°F)

415.4 °F - closed cup

Point d'éclair (°C)

213 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Wafa A Al-Megrin et al.
Frontiers in physiology, 11, 64-64 (2020-03-03)
The kidney is among the metabolic organs most susceptible to injury, particularly following exposure to xenobiotics and heavy metals. We aimed to explore the potential protective impacts of coenzyme Q10 (CoQ10) on lead acetate (PbAc)-induced nephrotoxicity in rats. Four experimental
Gadah Albasher et al.
Oxidative medicine and cellular longevity, 2020, 2963020-2963020 (2020-03-28)
Organophosphorus insecticides including chlorpyrifos (CPF) are mainly used for agriculture, household, and military purposes; their application is associated with various adverse reactions in animals and humans. This study was conducted to evaluate the potential neuroprotective effect of red beetroot methanolic
Alaa Jameel A Albarakati et al.
Molecular biology reports, 47(4), 2591-2603 (2020-03-08)
Lead (Pb) is one of the most common heavy metal pollutants affecting living organisms. It induces nephrotoxicity with significant alterations in renal structure and function. Luteolin (LUT) a flavonoid present in various plant products is well known for exhibiting numerous
S C Masters et al.
The Journal of biological chemistry, 276(48), 45193-45200 (2001-09-29)
The 14-3-3 proteins are a family of highly conserved eukaryotic regulatory molecules that play important roles in many biological processes including cell cycle control and regulation of cell death. They are able to carry out these effects through binding and
H Schroeter et al.
The Biochemical journal, 358(Pt 3), 547-557 (2001-09-06)
Oxidative stress has been associated with neuronal loss in neurodegenerative diseases and during age-associated cognitive decline. Flavonoids have been proposed to play a useful role in protecting the central nervous system against oxidative and excitotoxic stress, although the mechanism of
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