Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

View All Documentation

C8114

Sigma-Aldrich

Anti-α-E-Catenin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Numéro MDL:
MFCD05665180
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

IgG fraction of antiserum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 102 kDa

Espèces réactives

rat, human, canine

Technique(s)

immunocytochemistry: 1:200 using methanol-fixed, dog MDCK and human MCF7 cellc
microarray: suitable
western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line and cytosolic fraction of rat embryonic brain.

Numéro d'accès UniProt

P35221

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CTNNA1(1495)
rat ... Ctnna1(307505)

Description générale

α-E-Catenin is a predominant subtype of α-catenin. It is widely expressed but at low levels in the nervous system. Alternative spliced variants of α-E-Catenin include α1- and α2-E-catenin.
The catenins (α, β, γ) are cytoplasmic proteins found in varying abundance in many developing and adult tissues.

Immunogène

synthetic peptide corresponding to a region located near the C-terminus of human α-E-catenin (amino acids 873-887). This sequence is identical in mouse and Xenopus α-E-catenin. It is not found in α-N-catenin, β-catenin, and γ-catenin.

Application

Anti-α-E-Catenin antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Actions biochimiques/physiologiques

Catenins bind directly or indirectly to the conserved cytoplasmic tail domain of the cell adhesion adherins. The association of catenins to cadherins produces a complex, which is linked to the actin filament network. Catenins/cadherin complexes play an important role in mediating cell adhesion, transduction of cell-cell contact positional signals to the cell interior, and may play a crucial role in cell differentiation. The linkage of the epithelial cadherin /uvomorulin to actin is essential for the cell binding function of this cadherin. α-Catenin (CAP102, 102 kDa), originally described as an E-cadherin associated protein, has been shown to associate with other members of the cadherin family members, N-cadherin and P-cadherin. Within its conserved region α-catenin shows 30% identity to vinculin.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

p120 catenin is required for normal renal tubulogenesis and glomerulogenesis
Marciano DK, et al.
Development, 138(10), 2099-2109 (2011)
Jonathan Cohen et al.
The Journal of cell biology, 218(4), 1390-1406 (2019-03-15)
Development of the skin epidermis requires tight spatiotemporal control over the activity of several signaling pathways; however, the mechanisms that orchestrate these events remain poorly understood. Here, we identify a key role for the Wave complex proteins ABI1 and Wave2
Adnan Mahly et al.
BMC biology, 20(1), 145-145 (2022-06-17)
The establishment of tissue architecture requires coordination between distinct processes including basement membrane assembly, cell adhesion, and polarity; however, the underlying mechanisms remain poorly understood. The actin cytoskeleton is ideally situated to orchestrate tissue morphogenesis due to its roles in
Evolving form to fit function: cardiomyocyte intercalated disc and transverse-tubule membranes
Current Topics in Membranes, 72, 121-158 (2013)
A Nagafuchi et al.
Cell regulation, 1(1), 37-44 (1989-11-01)
Cadherins are a family of transmembrane glycoproteins which play a key role in Ca(2+)-dependent cell-cell adhesion. Cytoplasmic domains of these molecules are anchored to the cell cytoskeleton and are required for cadherin function. To elucidate how the function of cadherins
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique