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C6295

Sigma-Aldrich

Cell Freezing Medium-DMSO Serum free 1x

sterile-filtered, suitable for cell culture

Synonyme(s) :

cryopreservation medium

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

Impuretés

endotoxin, tested

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.

Application

Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.

Autres remarques

Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Xiangwen Zhan et al.
Biochemical and biophysical research communications, 501(3), 779-785 (2018-05-16)
The proto-oncogene PIM1 encodes Ser/Thr kinase and regulates cell growth, differentiation and apoptosis. However, more and more studies including ours have found that PIM1 can induce senescence in normal human diploid fibroblasts and behave as a tumor suppressor. But the
J Yang et al.
Oncogene, 36(34), 4828-4842 (2017-04-11)
PIM1 is a proto-oncogene, encoding a serine/threonine protein kinase that regulates cell proliferation, survival, differentiation and apoptosis. Previous reports suggest that overexpression of PIM1 can induce cellular senescence. However, the molecular mechanism underlying this process is not fully understood. Here
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
Afficher tous les articles scientifiques apparentés

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Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

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