C4407

Human Collagen Type III

from human placenta, powder, suitable for cell culture

• Numéro CAS: 9007-34-5
• UNSPSC Code: 12352202
• NACRES: NA.32
• EC Number: 232-697-4
• MDL number: MFCD00130825
• Form: powder
• Assay: ≥90% (GE)
• Biological source: human placenta

Propriétés

• Nom du produit: Collagène from human placenta, Bornstein and Traub Type III (Sigma Type X), powder
• biological source: human placenta
• Quality Segment: 200
• assay: ≥90% (GE)
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: HIV, hepatitis B and hepatitis C, none detected
• solubility: aqueous acid: soluble
• UniProt accession no.: Q03692
• storage temp.: 2-8°C
• Gene Information: human ... COL3A1(1300)

Description

Application

Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.

Preparation Note

This product was prepared by a modification of the pepsin extraction and salt fractionation method of Hill, R.J. and Harper, E., Anal. Biochem., 141 (1984). It is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

Le collagène se classe en un certain nombre de types structurellement et génétiquement distincts. Nous utilisons la nomenclature proposée par Bornstein et Traub. Il est important de ne pas confondre les désignations utilisées par Sigma et les types de classification reconnus.

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue.

Disclaimer

This product should be stored at 2-8 °C and will retain activity in these conditions for 2 years.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)