C2436

Cytochrome c from Saccharomyces cerevisiae

≥85% based on Mol. Wt. 12,588 basis

• Numéro CAS: 9007-43-6
• Numéro CE : 232-700-9
• Numéro MDL: MFCD00130890
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.61

Propriétés

• Source biologique: Saccharomyces cerevisiae
• Pureté: ≥85% based on Mol. Wt. 12,588 basis
• Forme: powder
• Poids mol.: 12,588 Da
• Technique(s): cell based assay: suitable
• Solubilité: water: 10 mg/mL, clear, red to red-brown (dark red)
• Numéro d'accès UniProt: P00044
• Application(s): cell analysis
• Température de stockage: −20°C
• Informations sur le gène: Saccharomyces cerevisiae ... CYC1(853507)

Description

Description générale

Cytochrome c (Cytc) comprises 104 amino acids and is a nuclear-encoded mitochondrial protein. This peripheral membrane protein is in spherical shape and has a molecular weight of 12,000 Da.

Application

Cytochrome c from Saccharomyces cerevisiae has been used in oocyte injection to induce caspase-3 activation and p38 MAPK (mitogen-activated protein kinase). It has also been used to analyze the aerobic reactions of nitric oxide.

Actions biochimiques/physiologiques

Cytochrome c (Cytc) participates in mitochondrial electron transport and intrinsic type II apoptosis. It plays a key role in aerobic energy production. This multi-functional enzyme serves as a single electron carrier. Cytc helps in the formation of the apoptosome.

The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. Tracking cellular localization of cytochrome c in S. cerervisiae demonstrates that mitochondria are strongly involved in programmed cell death, a process very similar to apoptosis in higher organisms.

Qualité

Care has been taken to maintain the native form of the protein. It has not been artificially oxidized or reduced during purification. Contains variable amounts of reduced cytochrome c.

Notes préparatoires

Prepared without using TCA.

Autres remarques

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)