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Merck

C-12620

Human Nasal Epithelial Cells (HNEpC)

500,000 cryopreserved cells

Synonyme(s) :

HNEpC cells

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A propos de cet article

NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human nose (nasal mucosa)
Morphology:
(epithelial)
Service technique
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biological source

human nose (nasal mucosa)

product line

PromoCell®

packaging

pkg of 500,000 cells

morphology

(epithelial)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

General description

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Application

Primary Human Nasal Epithelial Cells (HNEpC) are isolated from normal human nasal mucosa and are provided in a cryopreserved format. HNEpC stain positive for cytokeratin. The epithelium in the nasal cavity cleans, humidifies, and warms inhaled air. In addition, it produces mucus, which binds particles that are subsequently transported to the pharynx by cilia on the epithelial cells. HNEpC are useful for in vitro studies of these processes.

Biochem/physiol Actions

Nose

Preparation Note

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Analysis Note

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.


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Classe de stockage

12 - Non Combustible Liquids

Que se passe-t-il ?

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable



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