The specific site of biotin attachment on the IgG molecule has not been determined.
Additionally, the biotinylation procedure is proprietary and, therefore, cannot be disclosed.
B9183
Anticorps monoclonal Anti-HA
clone HA-7, purified from hybridoma cell culture
Synonyme(s) :
Anti-HA, Anti-hémagglutinine de la grippe
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| Conditionnement | Référence | Disponibilité | Prix |
|---|
A propos de cet article
Conjugate:
biotin conjugate
Clone:
HA-7, monoclonal
Application:
WB CL
Citations:
7
Le tarif et la disponibilité ne sont pas disponibles actuellement.
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.
shipped in
dry ice
storage temp.
2-8°C
General description
The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.
Immunogen
Peptide synthétique correspondant à un fragment de l′hémagglutinine (HA) du virus de la grippe humain, connu sous le nom d′étiquette HA ou HA-tag, conjugué à KLH.
Application
Antibody suitable for immunoblotting.
Biochem/physiol Actions
L′anticorps reconnaît tant les formes natives que dénaturées et réduites des protéines de fusion avec une étiquette HA. Il réagit avec les protéines de fusion exprimées dans E. coli ou dans les cellules de mammifères et dont l′étiquette HA se situe à l′extrémité N-terminale ou C-terminale.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
1 of 1
Cet article | |||
|---|---|---|---|
| antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin |
| conjugate biotin conjugate | conjugate unconjugated | conjugate alkaline phosphatase conjugate | conjugate FITC conjugate |
| clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C, 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
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Classe de stockage
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Contenu apparenté
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| B9183-100UG | 04061838069337 |
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Where is the biotin located on the IgG? Additionally, what protocol or method did you follow to biotinylate it?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
1 answer-
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
Helpful?
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