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Merck
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Key Documents

B8438

Sigma-Aldrich

Alcian Blue solution

1% in 3% acetic acid, pH 2.5

Synonyme(s) :

Alcian Blue 8XG solution

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About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Forme

liquid

Concentration

1% in 3% acetic acid, pH 2.5

Couleur

dark blue to very dark blue

Plage de pH

2.4-2.6

Amax

0.45-0.60 (1:400 in H2O)

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Application

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Actions biochimiques/physiologiques

Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW PH.
R LEV et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 12, 309-309 (1964-04-01)
Hao Wu et al.
Experimental cell research, 361(2), 324-332 (2017-11-04)
Mesenchymal stem-cell based therapies have been proposed as novel treatments for intervertebral disc (IVD) degeneration. The development of these treatment strategies, however, has been hindered by the incomplete understanding of the origin, biological properties of nucleus pulposus (NP) derived stem/progenitor
A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
Stain Technology, 51(2), 131-134 (1976)
Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.
J E Scott et al.
Histochemie. Histochemistry. Histochimie, 5(3), 221-233 (1965-10-01)
G F Pierce et al.
The American journal of pathology, 138(3), 629-646 (1991-03-01)
Recombinant platelet-derived growth factor (PDGF) and transforming growth factor beta 1 (TGF-beta 1) influence the rate of extracellular matrix formed in treated incisional wounds. Because incisional healing processes are difficult to quantify, a full-thickness excisional wound model in the rabbit
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