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APOAF

Sigma-Aldrich

Annexin V-FITC Apoptosis Detection Kit

Synonyme(s) :

Annexin V-FITC, Apoptosis probe FITC

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.84

Utilisation

(20 tests)

Niveau de qualité

300

Conditionnement

pkg of 1 kit

Technique(s)

flow cytometry: suitable

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).

Application

Annexin V-FITC Apoptosis Detection Kit was used:
  • in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.
  • for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.
  • for indirect measurement of flippase activity.

Caractéristiques et avantages

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Autres remarques

Allow all components to reach room temperature before use.

Composants de kit seuls

Réf. du produit
Description
  • APOAFA

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

08168

Timestrip Plus™ 8 °C

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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The Journal of biological chemistry, 287(18), 14912-14922 (2012-02-16)
Complementarity-determining regions (CDRs) from monoclonal antibodies tested as synthetic peptides display anti-infective and antitumor activities, independent of the specificity of the native antibody. Previously, we have shown that the synthetic peptide C7H2, based on the heavy chain CDR 2 from
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Flippases are key regulators of membrane asymmetry and secretory mechanisms. Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans. On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative
Ben-Zion Zaidman et al.
International journal of oncology, 31(4), 959-967 (2007-09-06)
Ganoderma lucidum (Curt.:Fr.) P. Karst, a medicinal fungus, has been widely used in Asian countries for centuries to prevent or treat a variety of diseases, including cancer. However, the mechanisms responsible for the effects of G. lucidum on cancer cells
Kalvin J Gregory et al.
PloS one, 5(10), e13428-e13428 (2010-10-27)
Vitamin D binding protein-macrophage activating factor (DBP-maf) is a potent inhibitor of tumor growth. Its activity, however, has been attributed to indirect mechanisms such as boosting the immune response by activating macrophages and inhibiting the blood vessel growth necessary for
Jing-Fang Hong et al.
Molecular medicine reports, 13(6), 4541-4548 (2016-04-16)
The aim of the present study was to investigate the in vitro and in vivo anticancer and apoptotic effects of taraxerol acetate in U87 human glioblastoma cells. The effects on cell cycle phase distribution, cell cycle-associated proteins, autophagy, DNA fragmentation and cell migration were
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