A9941

2,2′-azino-bis(acide 3-éthylbenzothiazoline-6-sulfonique) diammonium salt

chromogenic, tablet

• Synonyme(s) : 2,2′-azino-bis(3-éthylbenzothiazoline-6-sulfonate) de diammonium, AzBTS-(NH₄)₂
• Formule empirique (notation de Hill) : C₁₈H₂₄N₆O₆S₄
• Numéro CAS: 30931-67-0
• Poids moléculaire : 548.68
• NACRES: NA.21
• PubChem Substance ID: 24891410
• UNSPSC Code: 12352204
• EC Number: 250-396-6
• MDL number: MFCD00010404
• Beilstein/REAXYS Number: 7329461

Propriétés

• Nom du produit: 2,2′-azino-bis(acide 3-éthylbenzothiazoline-6-sulfonique) diammonium salt, tablet, 10 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• solubility: deionized water: 100 mL
• storage temp.: room temp
• SMILES string: [NH4+].[NH4+].CCN1C(\Sc2cc(ccc12)S([O-])(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
• InChI: 1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
• InChI key: OHDRQQURAXLVGJ-AXMZSLBLSA-N

Description

General description

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.
ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:

• For a reaction with laccase or bilirubin oxidase
• For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide

A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS).
ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound

Application

L'acide 2,2′-azino-bis(3-éthylbenzothiazoline-6-sulfonique) est un substrat de la peroxydase qui peut être utilisé dans les protocoles ELISA. Il permet d'obtenir un produit final de couleur verte soluble, mesurable par spectrophotométrie à 405 nm. La réaction peut être arrêtée par l'ajout de dodécylsulfate de sodium (SDS) à 1 %. Ce substrat est recommandé pour les protocoles ELISA (micropuits) ; il n'est pas recommandé pour les techniques à membrane.

• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†
• ABTS is also used to measure glucose concentrations in different solutions including blood serum.
• The food industry uses ABTS to measure the antioxidant capacities within different foods.
• Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results
• It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Preparation Note

Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves
• wgk: WGK 2
• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2