A9594

Anticorps monoclonal ANTI-FLAG^® M2 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

• Synonyme(s) : Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 41106514
• Conjugate: CY3 conjugate
• Clone: M2, monoclonal
• Application: IF (d)
• Citations: 42

Propriétés

• biological source: mouse
• Quality Segment: 200
• conjugate: CY3 conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
• species reactivity: all
• concentration: ~1 mg/mL
• technique(s): direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Learn more product details in our FLAG^® application portal.

Physical form

Solution in phosphate buffered saline plus 1% BSA and preservative

Preparation Note

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

Other Notes

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Cy3 is a trademark of Cytiva

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable