A8011
Antifoam C Emulsion
aqueous-silicone emulsion
Synonyme(s) :
Antifoaming Agent Emulsion
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About This Item
Code UNSPSC :
12161900
Nomenclature NACRES :
NA.25
Produits recommandés
Source biologique
synthetic
Niveau de qualité
Forme
emulsion
Contient
emulsifier
Technique(s)
cell culture | hybridoma: suitable
microbiological culture: suitable
Pf
0 °C (32 °F)
Catégories apparentées
Description générale
Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.
Application
Antifoam C emulsion has been used:
- as a supplement in RO-H2O and a chemically defined culture medium to determine volumetric mass transfer coefficient (kLa) of mammalian cell culture
- as a component of feed medium,
- as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
- as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions
Caractéristiques et avantages
- Minimizes compatibility issues with biological systems
- Highly effective defoamer at low concentrations
- Highly effective and versatile silicone defoamer
Autres remarques
A 30% aqueous emulsion of polydimethylsiloxane concentrate.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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In aerobic cell cultivation processes, dissolved oxygen is a key process parameter, and an optimal oxygen supply has to be ensured for proper process performance. To achieve optimal growth and/or product formation, the rate of oxygen transfer has to be
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Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
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American Pharmacy null
Mervyn W O Liew et al.
Journal of biotechnology, 150(2), 224-231 (2010-08-28)
This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
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