A5384

Aconitase from porcine heart

• Synonyme(s) : Aconitate hydratase, Citrate (isocitrate) hydrolyase
• Numéro CAS: 9024-25-3
• Numéro CE : 4.2.1.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• MDL number: MFCD00130335
• Biological source: Porcine heart

Propriétés

• biological source: Porcine heart
• Quality Segment: 200
• form: solid
• mol wt: ~66 kDa by gel filtration
• storage temp.: −20°C

Description

Application

Aconitase may be usefull in diabetes research as well as to study structural conservation between iron-responsive element binding proteins (IRE-BP) and isomerases .

Biochem/physiol Actions

Aconitase catalyses the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle. Aconitase is inhibited by cyanide, sulfide and copper and mercury at low concentrations. It is competitively inhibited by trans-aconitate. Aconitase contains an internal Fe-S cluster which is responsible for its activity.

Analysis Note

Crude preparation containing aconitase activity

Other Notes

One unit will convert 1.0 μmole of citrate (via cis-aconitate) to isocitrate per min at pH 7.4 at 25 °C.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)