Sybr Green and Fluorescein sodium salt are both green dyes, and although their spectral ranges are close, it is important to consider their specific uses and characteristics. While Sybr Green is a popular dye for molecular use, Fluorescein sodium salt is a very economical product and may be useful for specific applications. However, there is no information comparing the two dyes for use in PCR.
46960
Fluorescein sodium salt
BioReagent, suitable for fluorescence
Synonyme(s) :
Acid Yellow 73, NaFl, Sodium fluorescein, Yellow No. 8, D&C;Yellow No. 8, NaFluo, Uranine
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A propos de cet article
Formule empirique (notation de Hill) :
C20H10Na2O5
Numéro CAS:
Poids moléculaire :
376.27
EC Number:
208-253-0
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3833041
Colour Index Number:
45350
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BioReagent
Quality Segment
form
powder
technique(s)
titration: suitable
fluorescence
λex 460 nm; λem 515 nm(lit.), λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
SMILES string
[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
InChI key
RGPLGPBQJOQFJS-UHFFFAOYSA-L
General description
Fluorescein Sodium Salt (FSS) is a xanthene dye having a polycrystalline structure with a monoclinic system. Fluorescein is a synthetic organic compound available as a dark orange/red powder soluble in water and alcohol.
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|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| technique(s) titration: suitable | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - |
| fluorescence λex 460 nm; λem 515 nm(lit.), λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0 | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence - |
| form powder | form powder | form - | form powder |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| product line BioReagent | product line - | product line - | product line - |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
423.7 °F - Pensky-Martens closed cup
flash_point_c
217.6 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves, type N95 (US)
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For Fluorescein sodium salt 46960, is there a fluorescence value for a positive PCR sample, and how would this value translate as a concentration of this powder?
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