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Merck

43354

Abberior® STAR RED NHS

≥80.0% (degree of coupling)

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A propos de cet article

NACRES:
NA.32
UNSPSC Code:
12352108
Service technique
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Quality Segment

assay

≥80.0% (degree of coupling)

fluorescence

λex 637 nm; λem 660 nm±10 nm in water

storage temp.

−20°C

General description

Abberior STAR RED is an already well known dye named also KK114 in literature (a scientific web search shows more than 60 publications, a selection is listed below). Please note the minimal background signal due to negligible unspecific binding. A key feature of the dye is its comparably long lifetime of ~3.5ns. The dye works exceptionally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope.
Abberior STAR RED can substitute Atto 647N, AlexaFluor 647, or Cy®5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference [2] for detailed characteristics.
Best results are obtained with freshly prepared samples.

Application

Abberior® STAR RED labelled cholesterol analogs were used for STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) based measurements of membrane diffusion dynamics. Anti-mouse secondary antibody coupled to Abberior® STAR RED has been used for STED microscopy imaging in Vero cell line.
Designed and tested for fluorescent super-resolution microscopy

Legal Information

Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH


Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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