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Key Documents

31149

Sigma-Aldrich

Deoxyribonucleic acid, low molecular weight from salmon sperm

Synonyme(s) :

Salmon sperm dsDNA, low molecular weight DNA, DNA

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About This Item

Numéro CAS:
100403-24-5
Numéro CE :
309-566-6
Numéro MDL:
MFCD00130921
Code UNSPSC :
41106310
eCl@ss :
32160414
Nomenclature NACRES :
NA.52

Qualité

for molecular biology
puriss. p.a.

Forme

lyophilized powder

Impuretés

≤5% protein

Solubilité

50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow

Rapport des absorbances

A260/280 nm ~1.4

Température de stockage

2-8°C

Application

Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
  • to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.
  • in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.
  • as a precursor in the synthesis of the bio-dots.

Actions biochimiques/physiologiques

Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Thiol modification of silicon-substituted hydroxyapatite nanocrystals facilitates fluorescent labelling and visualisation of cellular internalisation
Williams RL et al
Journal of Material Chemistry B: Materials for Biology and Medicine, 35, 4370-4378 (2013)
V N Kondratova et al.
Biochemistry. Biokhimiia, 74(11), 1285-1288 (2009-11-18)
A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters
Vyacheslav Palchevskiy et al.
Journal of bacteriology, 188(11), 3902-3910 (2006-05-19)
Natural genetic competence is the ability of cells to take up extracellular DNA and is an important mechanism for horizontal gene transfer. Another potential benefit of natural competence is that exogenous DNA can serve as a nutrient source for starving
J Wang et al.
FEBS letters, 351(2), 219-224 (1994-09-05)
The Ku antigen consists of 70 and 80 kDa protein subunits (p70 and p80, respectively) that form the DNA binding component of a DNA-dependent protein kinase (DNA-PK). It is controversial whether the interaction of Ku with DNA is mediated by
Sébastien Balme et al.
Soft matter, 13(2), 496-502 (2016-12-09)
The fundamental understanding of the transport mechanisms of objects across a single nanopore is one key point to develop Coulter counters at the nanoscale for macromolecule or nanoparticle detection. In this area, nanoparticles have been less investigated than biomacromolecules such
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