03978

Methylene Blue solution

concentrate according to Ehrlich, concentrated, aqueous solution

• Synonyme(s) : Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Formule empirique (notation de Hill) : C₁₆H₁₈ClN₃S
• Numéro CAS: 61-73-4
• Poids moléculaire : 319.85
• NACRES: NA.47
• PubChem Substance ID: 329747853
• UNSPSC Code: 12171500
• MDL number: MFCD00012111
• Beilstein/REAXYS Number: 3641570

Propriétés

• Quality Segment: 100
• form: liquid
• quality: concentrated, aqueous solution
• shelf life: ~3 yr
• concentration: 1.5% (1.5 g/ 100 mL)
• density: 1.0 g/mL at 20 °C
• εmax: 9.4 at 656-666 nm in water
• suitability: suitable for microscopy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

General description

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Informations sur la sécurité

• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
• Classe de stockage: 12 - Non Combustible Liquids