E4884

Acide éthylènediaminetétraacétique disodium salt dihydrate

ACS reagent, 99.0-101.0%

• Synonyme(s) : EDTA disodium salt, EDTA-Na₂, Edétate disodium salt dihydrate, Sequestrene Na₂, Édathamil, Éthylènediaminetétraacétate disodique dihydrate
• Formule empirique (notation de Hill): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• Numéro CAS: 6381-92-6
• Poids moléculaire : 372.24
• Numéro Beilstein : 3900609
• Numéro CE : 205-358-3
• Code UNSPSC : 12352107
• ID de substance PubChem : 24894586
• Nomenclature NACRES : NA.77

Propriétés

• Qualité: ACS reagent
• Niveau de qualité: 200
• Agence: suitable for SM 4500 - NH3
• Pureté: 99.0-101.0%
• Forme: solid
• Pertinence de la réaction: reagent type: chelator
• Impuretés: ≤0.005% Insoluble matter; ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)
• pH: 4.0-6.0 (25 °C, 5%)
• Pf: 248 °C (dec.) (lit.)
• Solubilité: H₂O: 100 g/L at 20 °C
• Traces de cations: Fe: ≤0.01%; heavy metals: ≤0.005% (by ICP-OES)
• Application(s): microbiology
• Température de stockage: room temp
• Chaîne SMILES: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• Clé InChI: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

Description générale

Ethylenediaminetetraacetic acid disodium salt dihydrate or EDTA, is a chelating agent with many applications in molecular biology. EDTA is often used in buffers as a metal ion inhibitor to maintain the stability and integrity of biological samples. EDTA binds to metal cations by forming a stable octahedral complex. Its ability to chelate metal ions makes it extremely useful for blocking metal-dependent enzymatic activities, such as DNases and proteases. Additionally, EDTA is often found in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Application

Antioxidant assays (1) EDTA is also employed in molecular biology techniques, such as DNA extraction, purification, and electrophoresis, where it helps to prevent metal-mediated degradation of nucleic acids. Additionally, EDTA is utilized in cell culture media as a sequestrant to bind and neutralize metal ions present in the medium, thereby promoting cell growth and viability.

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

• of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
• of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
• of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Chélateur de cations divalents. Inhibe les enzymes dont l′activité exige des cations divalents, comme les métalloprotéinases.

Caractéristiques et avantages

American Chemical Society (ACS) grade is suitable for use in standard analytical methods

Autres remarques

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Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H332,H373
• Conseils de prudence: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Classification des risques: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Organes cibles: Respiratory Tract
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)