XGAL-RO

5-bromo-4-chloro-3-indolyl β-D-galactopyranoside

solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

• Synonyme(s) : 5-bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
• Formule empirique (notation de Hill) : C₁₄H₁₅BrClNO₆
• Numéro CAS: 7240-90-6
• Poids moléculaire : 408.63
• UNSPSC Code: 12352204
• NACRES: NA.28
• PubChem Substance ID: 329830810
• MDL number: MFCD00005666
• Beilstein/REAXYS Number: 1402009
• Assay: 99%
• Concentration: 20 mg/mL

Propriétés

• description: 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
• assay: 99%
• form: solid
• mol wt: 408.63 g/mol
• packaging: pkg of 1 g (10745740001), pkg of 100 mg (03117073001), pkg of 100 mg (11680293001), pkg of 2.5 g (10703729001), pkg of 250 mg (10651745001)
• manufacturer/tradename: Roche
• concentration: 20 mg/mL
• technique(s): activity assay: suitable
• color: white
• solubility: DMF: soluble, methanol: soluble
• application(s): genomic analysis; life science and biopharma
• storage temp.: 2-8°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• InChI key: OPIFSICVWOWJMJ-AEOCFKNESA-N

Description

General description

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Application

X-Gal est un substrat chromogène pour la β-galactosidase qui produit une riche couleur bleue facilement détectable visuellement par rapport au fond. X-Gal est le substrat de choix pour la sélection bleu-blanc des colonies bactériennes recombinantes avec le génotype lac⁺.

X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

Physical form

Crystals, white powder

Preparation Note

Working solution: Stock solution:

• 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
• A_{590 nm}: 0.01

Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.

Analysis Note

Chromatographically homogeneous

Other Notes

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable