TR-1008
Sodium Butyrate Solution
This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.
Synonyme(s) :
Cell Culture NaBu
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About This Item
Code UNSPSC :
12352207
eCl@ss :
32160801
Nomenclature NACRES :
NA.75
Produits recommandés
Niveau de qualité
Forme
liquid
Fabricant/nom de marque
Specialty Media
Entrée
sample type: mouse embryonic stem cell(s)
Description générale
This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.
Application
Sodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.
This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.
This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.
Stockage et stabilité
Store at 2-8°C for 2 years.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Eye Dam. 1 - Skin Corr. 1B
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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