MABE419

Anti-m3G-cap, m7G-cap Antibody, clone H-20

clone H-20, from mouse

• Synonyme(s) : Anti-m3G-cap, clone H-20, Anti-m3G/m7G-cap, Anticorps de détection anti-m7G-ca
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: H-20, monoclonal
• Technique(s): dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• Application: DB, ICC, IP, WB
• Citations: 8

Propriétés

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: H-20, monoclonal
• species reactivity (predicted by homology): all
• technique(s): dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

The nucleotide structure named the 2,2,7-trimethylguanosine(m3G)-containing cap structure of small nuclear ribonucleoprotein particles, or U snRNPs is an essential part of mRNA processing. Each snRNP particle consists of one (U1, U2 and U5) or two (U4/U6) snRNA molecules, a common set of eight core proteins (B, B9, D1, D2, D3, E, F and G, also denoted Sm proteins) that are bound to each of the 2,2,7- trimethylguanosine (m3G) cap-containing snRNAs U1, U2, U4 and U5, and several proteins associated specifically with the individual U snRNPs. Except for U6 snRNP, which does not leave the nucleus, the synthesis of these U snRNPs requires the bidirectional transport of the snRNA across the nuclear envelope. The snRNAs U1, U2, U4 and U5 are synthesized in the nucleus with a 7-monomethylguanosine (m7G) cap structure whereas the Sm proteins are stored in the cytoplasm and do not migrate into the nucleus in the absence of bound U snRNA. ’ Stable association of all Sm proteins is essential for the hypermethylation of the m7G-cap to the m3G-cap structure. After this event and processing of the snRNAs, the mature snRNP particles are transported back to the nucleus in a receptor-and energy-dependent manner and the complete particle is formed. Monoclonal H-20 recognizes both m3G cap containing snRNPs as well as m7G capped structures and it should have a wide application, not only for studying the molecular biology and immunology of the U snRNPs from diverse organisms, but also for the characterization and isolation of m7G-capped transcripts.

Immunogen

Human m3G-cap and m7G-cap

Application

&

Analyse par immunoprécipitation : µ

A representative lot from an independent laboratory detected m3G-cap in m3G-capped snRNAs from HeLa nuclear extracts (Bochnig, P., et al. (1987).

A representative lot from an independent laboratory immunoprecipitated m3G-cap from prefractionated HeLa S100 extracts (Huber, J., et al. (1998).

A representative lot from an independent laboratory detected m3G-cap in m3G-capped U1 snRNPs from digitonin permeabilized HeLa cells (Bochnig, P., et al. (1987). Eur J Biochem. 168(2):461-467.).

Domaine de recherche
Épigénétique et fonction nucléaire

Sous-domaine de recherche
Biologie de la chromatine

Physical form

κ

Format : Produit purifié

Produit purifié sur protéine G

Preparation Note

Stable à -20 °C pendant 1 an à compter de la date de réception.
Recommandations relatives à la manipulation du produit : dès réception, et avant le retrait du bouchon, centrifuger le flacon et mélanger délicatement la solution. Répartir en aliquotes dans des microtubes à centrifuger et conserver ces derniers à -20 °C. Éviter les congélations/décongélations répétées, qui peuvent détériorer les IgG et nuire aux performances du produit.

Analysis Note

µ

Other Notes

Concentration : pour connaître la concentration spécifique du lot, voir le certificat d'analyse.

Disclaimer

Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.

Informations sur la sécurité

• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable