HC08

Fluorescent Mounting Media, Aqueous

This Fluorescent Mounting Media, Aqueous is validated for use in Immunofluorescence for the detection of Fluorescent Mounting Media Aqueous.

• NACRES: NA.47
• UNSPSC Code: 41116133

Propriétés

• form: liquid
• does not contain: preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

An aqueous mounting medium designed to preserve and enhance the labeling of immunoreactivity in tissue sections. Sustains the fluorescent signal from labeled samples on slides. Inclusion of 4,6-diamidino-2-phenylindole (DAPI; Cat. No. 268298) allows for the visualization of unlabeled (non-apoptotic) cells. Suitable for use with a wide variety of fluorophores.

Aqueous mounting media designed to preserve and enhance the labeling of tissue sections or cell preparations fixed on slides. Suitable for many fluorophores including fluorescein, Oregon Green, Alexa 488, rhodamine, Texas Red, Cyanine (Cy) 3, and Alexa 546. Inclusion of DAPI in the Fluorescent Mounting Media allows for the visualization of all cells at an excitation wavelength of 330-380 nm.

Application

Immunofluorescence (see comments)

Physical form

8 mL in glycerol solution containing 25 mg/ml 1,4-DiAzaBicyclo-[2.2.2]Octane (DABCO) in Tris buffer and 0.1 µg/ml 4,6-DiAmidino-2-PhenylIndole (DAPI).

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

For best results: following the final aqueous step of the staining procedure remove excess fluid around the specimen, apply 1-2 drops of Fluorescent Mounting Media to the specimen and carefully place a coverglass on top. Seal the border of the coverglass with nail polish. Store mounted specimens at 4°C in the dark.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Classe de stockage: 12 - Non Combustible Liquids
• wgk: WGK 1