GF315

Vascular Endothelial Growth Factor 165 human

animal free, >98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, suitable for cell culture

• Synonyme(s) : Vascular Endothelial Growth Factor 165, VEGF-165, VEGF, Vascular Endothelial Growth Factor, VEGF-A
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.77
• Assay: >98% (SDS-PAGE and HPLC)
• Impurities: <0.1 μg/mg protein endotoxin (<1EU/μg)

Propriétés

• Nom du produit: VEGF165 Protein, Human Recombinant Animal Free, The Vascular endothelial growth factor (VEGF or VEGFA) is a potent mediator of both angiogenesis & vasculogenesis in the fetus & adult. Manufactured using all non-animal reagents.
• Quality Segment: 100
• assay: >98% (SDS-PAGE and HPLC)
• impurities: <0.1 μg/mg protein endotoxin (<1EU/μg)
• NCBI accession no.: NM_001025366.1, NM_001025367.1, NM_001025368.1, NM_001025369.1, NM_001025370.1, NM_001033756.1, NM_003376.4
• UniProt accession no.: P15692
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Product Source: E. coli

Vascular endothelial growth factor (VEGF or VEGFA) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant human VEGF is a 38.2 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Cross Reactivty
Mouse, Rat, Pig, Hamster, Trout, Green Algae, Sheep, Cow, Rabbit, Guinea Pig

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human VEGF165 is manufactured using all non-animal reagents

Preparation Note

Store at -20°C for up to 6 months from date of receipt.

Analysis Note

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• WGK: WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable