GF312

SCF Protein, Human Recombinant Animal Free

The Stem cell factor also known as ckit ligand, mast cell growth factor & steel factor (SLF), is a widely expressed 28-40kDa type I transmembrane glycoprotein. Recombinant animal free human SCF is manufactured using all non-animal reagents.

• Synonyme(s) : Stem Cell Factor, ckit Ligand, Mast Cell Growth Factor, Stell Factor, MGF
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405
• Assay: >98% (SDS-PAGE and HPLC)
• Recombinant: expressed in E. coli

Propriétés

• recombinant: expressed in E. coli
• Quality Segment: 100
• assay: >98% (SDS-PAGE and HPLC)
• impurities: <0.1 μg/mg protein endotoxin (<1EU/μg)
• NCBI accession no.: NM_000899.3, NM_003994.4
• UniProt accession no.: P21583
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Product Source: E. coli

Stem cell factor (SCF), also known as ckit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Cross Reactivty
Monkey

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human SCF is manufactured using all non-animal reagents

Preparation Note

Store at -20°C for up to 6 months from date of receipt.

Analysis Note

Bioactivity assay: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 1