AG782
Angiotensin Converting Enzyme, rat
Synonyme(s) :
ACE, CD143, DCP1, Peptidyl-dipeptidase A, Peptidase P
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
A propos de cet article
UNSPSC Code:
12352204
NACRES:
NA.42
eCl@ss:
32160405
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderGeneral description
ACE (kininase II, EC 3.4.15.1) is a zinc dipeptidyl carboxypeptidase. ACE catalyses both angiotensin I transformation to angiotensis II (a potent vasopressor agent) and degradation of bradikinin, a vaso-depressor. ACE appears to play a key role in regulating vascular tone and remodeling the development of atherosclerotic lesions. Increasing evidence suggests that ACE plays an important role in vascular pathology. Recent studies shows two new functions of this important enzyme: 1) It enhances the presentation of endogenous antigens (in particularly HIV 1 gp160-derived peptide p18) to MHC class I-restricted T-lynphocytes (ref. 1); 2) Its amino-terminal domain cleaves effectively hemoregulatory tetrapeptide N-Ac-Ser-Asp-Lys-Pro (ref. 2,3) which is involved in the control of hemapoetic stem cell proliferation (the amount of this peptide greatly decreases in patients undergoing cancer chemotherapy) (ref. 4).
Product Source: Isolated from rat lung using affinity chromatography (ref. 5).
Application
As a reference in SDS-PAGE and Western blotting [together with anti-ACE monoclonal (MAB4051) also available from Chemicon]. For Western blotting 500 ng/lane if gel is developed with anti-ACE MAB4051.
As a reference antigen for immunoprecipitation of ACE from various tissue samples (in combination with anti-ACE MAB4051).
As a source of pure ACE for different enzymatic studies of ACE (testing of ACE inhibitors, substrate specificity, kinetic measurements, etc.).
Optimal working concentration must be determined by the end user.
As a reference antigen for immunoprecipitation of ACE from various tissue samples (in combination with anti-ACE MAB4051).
As a source of pure ACE for different enzymatic studies of ACE (testing of ACE inhibitors, substrate specificity, kinetic measurements, etc.).
Optimal working concentration must be determined by the end user.
Physical form
Liquid in 100 mM phosphate buffered saline, 150 mM NaCl, pH 7.4 with 0.2mM CHAPS. Purity is >95% by SDS-PAGE when 10 μg was loaded. Contains no preservative.
Other Notes
Concentration: Refer to Certificate of Analysis for each individual lot of this enzyme.
Specific Activity: One unit will produce 1 μmoL of hippuric acid or His-Leu from Z-Phe-His-Leu per minute in 0.1 M phosphate buffer and 300 mM NaCl at pH 8.3 at 37°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Classe de stockage
12 - Non Combustible Liquids
Que se passe-t-il ?
WGK 2
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.