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Key Documents

ABE44-S

Sigma-Aldrich

Anti-trimethyl Histone H3 (Lys27) Antibody, Trial Size

from rabbit, purified by affinity chromatography

Synonyme(s) :

H3K27me3, Histone H3 (trimethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3, H3K27me3

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human, mouse

Technique(s)

dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable

Numéro d'accès NCBI

NP_003484.1

Numéro d'accès UniProt

Q16695

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

trimethylation (Lys27)

Informations sur le gène

human ... HIST1H3F(8968)

Description générale

Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.

Spécificité

Broad species cross-reactivity expected.

Application

Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27 (H3K73me). This purified Ab is specificity verified by dot blot (DB) & validated in WB, ICC, PIA. Small pack size is ideal for screening applications.

Qualité

Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.

Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .

Description de la cible

~ 17 kDa

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Danyang Li et al.
International journal of biological sciences, 15(1), 69-80 (2019-01-22)
EZH2 plays vital roles in epigenetic regulation, neuronal development and cancer progression. Here a novel EZH2 variant, namely EZH2-X9 (X9 for short) resulting from alternative splicing, was isolated, identified and functionally characterized. X9 was highly expressed in the brains of
Shumeng Liu et al.
Molecular cell, 67(5), 853-866 (2017-08-15)
Lysine crotonylation (Kcr) is a newly identified histone modification that is associated with active transcription in mammalian cells. Here we report that the chromodomain Y-like transcription corepressor CDYL negatively regulates histone Kcr by acting as a crotonyl-CoA hydratase to convert
Huiming Chen et al.
Oncotarget, 6(15), 13049-13059 (2015-05-07)
Polycomb repressive complex 2 (PRC2), which is responsible for the trimethylation of H3K27 (H3K27me3), plays a part in tumorigenesis, development and/or maintenance of adult tissue specificity. The pivotal role of PRC2 in cancer makes it a therapeutic target for epigenetic

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