524734

Phosphodiesterase 3B, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonyme(s) : PDE3B, cGMP-inhibited phosphodiesterase 3B
• NACRES: NA.77
• UNSPSC Code: 12352202
• Specific activity: ≥0.5 U/μL

Propriétés

• form: liquid
• Quality Segment: 100
• specific activity: ≥0.5 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full-length, recombinant, human phosphodiesterase 3B (accession no. AY459346) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. An important component of insulin and cAMP-dependent signaling. Supplied as a partially purified preparation.

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.
Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.

One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• Classe de stockage: 10 - Combustible liquids
• Que se passe-t-il ?: WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable