324796

Lys-C Protease

Cleaves at lysine residues, suitable for mass spectrometry, from Achromobacter lyticus

• Synonyme(s) : Endopeptidase Lys-C, Achromobacter lyticus
• Numéro CAS: 78642-25-8
• MDL number: MFCD00130333
• UNSPSC Code: 12352202
• NACRES: NA.54
• Specific activity: ≥2 AU/mg protein

Propriétés

• Nom du produit: Endopeptidase Lys-C, Achromobacter lyticus,
• form: lyophilized
• Quality Segment: 100
• specific activity: ≥2 AU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• technique(s): mass spectrometry (MS): suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. Optimal pH: 9.0-9.5.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Reconstitute 1 vial with 1 ml solvent; if the enzyme is not completely solubilized, add 1-3 ml additional solvent until completely dissolved.

Other Notes

Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

One AU (amidase unit) is defined as the amount of enzyme that catalyzes the release of 1 µM pNA from N-benzoyl-DL-lysine-pNA per min at 30°C, pH 9.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Informations sur la sécurité

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 2