219461
Cathepsin K, His•Tag®, Human, Recombinant, E. coli
Cathepsin K, His•Tag, Human, Recombinant, E. coli, CAS 94716-09-3, of the papain superfamily of cysteine proteinases plays a role in osteoclast-mediated bone resorption and collagen degradation.
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A propos de cet article
Numéro CAS:
NACRES:
NA.77
UNSPSC Code:
12352202
MDL number:
Form:
liquid
Assay:
≥95% (SDS-PAGE)
Recombinant:
expressed in E. coli
Service technique
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Laissez-nous vous aiderrecombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
form
liquid
specific activity
≥900 mU/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
Cathepsin K is a member of the papain superfamily of cysteine proteinases and plays an important role in osteoclast-mediated bone resorption and collagen degradation. It is primarily expressed by osteoclasts and recent studies also suggest its expression in fibroblasts. Irregularities in cathepsin K activity, either too high or too low, contribute to osteopenic disorders such as osteoporosis and pycnodysostosis. This recombinant enzyme contains a C-terminal His•Tag sequence.
Note: 1 mU = 1 milliunit.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM NaOAC, pH 5.5. The activation was stopped by addition of 10 mM MMTS, which can be removed under reducing conditions.
Preparation Note
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Delaisse, J.M., et al. 2003. Microsc. Res. Tech.61, 504.
Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.
Hou, W.S., et al. 2003. Biol. Chem.384, 891.
Dodds, R.A. 2003. Cell Biochem. Funct.21, 231.
Everts, V., et al. 2003. Calcif. Tissue Int.73, 380.
Hou, W.S., et al. 2003. Biol. Chem.384, 891.
One unit is defined as the amount of enzyme that will hydrolyze 0.4 µmol Z-FR-AMC per min at 37°C, pH 5.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Classe de stockage
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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