203980

α-Bungarotoxin

from Bungarus multicinctus, lyophilized solid, irreversible binder to motor end-plate acetylcholine receptor, Calbiochem®

• Synonyme(s) : α-Bungarotoxin, Bungarus multicinctus
• Formule empirique (notation de Hill) : C₃₃₈H₅₂₉N₉₇O₁₀₅S₁₁
• Numéro CAS: 11032-79-4
• Poids moléculaire : 7984.12
• MDL number: MFCD00007185
• UNSPSC Code: 12352200
• NACRES: NA.77
• Form: lyophilized solid
• Storage condition: OK to freeze

Propriétés

• Nom du produit: α-Bungarotoxin, Bungarus multicinctus, from Bungarus multicinctus, lyophilized solid, irreversible binder to motor end-plate acetylcholine receptor, Calbiochem®
• Quality Segment: 100
• description: Merck USA index - 14, 1488
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: 95% (HPLC)
• solubility: PBS: soluble, water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A polypeptide composed of 74 amino acids containing 5 disulfide bridges. Blocks neuromuscular transmission by irreversible binding to the motor end-plate acetylcholine receptor (K_d = 1 nM to 1 pM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced augmentation in intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM). Prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-Bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

Blocks neuromuscular transmission by irreversible binding to motor end-plate acetylcholine receptor (K_d = 1 pM to 1 nM) but does not depress acetylcholine release from motor nerve endings. Blocks nicotine-induced increase of intracellular Ca²⁺ in PC12 cells (IC₅₀ = 310 nM), and prevents opening of nicotinic receptor-associated ion channels. Reconstitution experiments in Xenopus oocytes have shown the effects of α-bungarotoxin on neuronal nAChR to be highly specific for the α₇-subtype (IC₅₀ = 1.6 nM), but not for the α₃β₄-subtype (IC₅₀ >3 µM).

Biochem/physiol Actions

Cell permeable: no

Kd = 1 pM to 1 nM for motor end-plate acetylcholine receptor

Primary Target
Motor end-plate acetylcholine receptor

Product does not compete with ATP.

Reversible: no

Physical form

Supplied as an acetate salt

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

H-Ile-Val-Cys³-His-Thr-Thr-Ala-Thr-Ser-Pro-Ile-Ser-Ala-Val-Thr-Cys¹⁶-Pro-Pro-Gly-Glu-Asn-Leu-Cys²³-Tyr-Arg-Lys-Met-Trp-Cys²⁹-Asp-Ala-Phe-Cys³³-Ser-Ser-Arg-Gly-Lys-Val-Val-Glu-Leu-Gly-Cys⁴⁴-Ala-Ala-Thr-Cys⁴⁸-Pro-Ser-Lys-Lys-Pro-Tyr-Glu-Glu-Val-Thr-Cys⁵⁹-Cys⁶⁰-Ser-Thr-Asp-Lys-Cys⁶⁵-Asn-Pro-His-Pro-Lys-Gln-Arg-Pro-Gly-OH (disulfide bonds: 3 → 23; 16 → 44; 29 → 33; 48 → 59; 60 → 65)

Lopez, M.G., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 14184.
Zhang, Z.W., et al. 1994. Neuron 12, 167.
Bambrick, L.L., and Gordon, T. 1992. J. Physiol.449, 479.
Lin, S.R., and Chang, C.C. 1992. Biochim. Biophys. Acta1159, 255.
Motomura, M., et al. 1992. Neurosci. Lett.143, 139.
Sorenson, E.M., and Chiappinelli, V.A. 1992. J. Comp. Neurol.323, 1.
Ruan, K.H., et al. 1990. Proc. Natl. Acad. Sci. USA87, 6156.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• Que se passe-t-il ?: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable