203391

(−)-Blebbistatin

≥98% (HPLC), solid, ATPase inhibitor, Calbiochem^®

• Synonyme(s) : (-)-Blebbistatin
• Formule empirique (notation de Hill) : C₁₈H₁₆N₂O₂
• Numéro CAS: 856925-71-8
• Poids moléculaire : 292.33
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD08460907
• Assay: ≥98% (HPLC)
• Form: solid
• Storage condition: OK to freeze, protect from light

Propriétés

• Nom du produit: (-)-Blebbistatin, The active enantiomer of (±)-Blebbistatin that accounts for the inhibitory activity towards ATPase and myosin II-dependent cellular processes.
• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: solid
• potency: 2 μM IC₅₀
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellow
• solubility: methanol: 1.5 mg/mL, 100% DMSO: 100 mg/mL, 90% DMSO: 75 mg/mL
• shipped in: wet ice
• storage temp.: −20°C
• SMILES string: N2(CC[C@@]3(C2=Nc4c(cc(cc4)C)C3=O)O)c1ccccc1
• InChI: 1S/C18H16N2O2/c1-12-7-8-15-14(11-12)16(21)18(22)9-10-20(17(18)19-15)13-5-3-2-4-6-13/h2-8,11,22H,9-10H2,1H3/t18-/m1/s1
• InChI key: LZAXPYOBKSJSEX-GOSISDBHSA-N

Description

General description

The active enantiomer of (±)-Blebbistatin (Cat. No. 203390) that accounts for the inhibitory activity towards ATPase (IC₅₀ = ~2 µM) and myosin II-dependent cellular processes. Also reported to potently inhibit the actomyosin ATPase activities of expressed smooth muscle myosin IIA (SMA) and smooth muscle myosin IIB (SMB) smooth muscle myosin II heavy-chain isoforms (IC₅₀ ~3 µM).

Biochem/physiol Actions

Product does not compete with ATP.

Reversible: yes

Cell permeable: yes

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month in 90% DMSO or methanol (-20°C). Stock solutions in 100% DMSO are unstable; reconstitute just prior to use.

Other Notes

Eddinger, T.J. et al. 2007. J. Pharmacol. Exp. Ther.320, 865.
Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Informations sur la sécurité

• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 3