1.12374

Thrombin

from bovine plasma, 50 NIH units/mg dry wt

• Synonyme(s) : Thrombin, Coagulation factor IIa, Fibrinogenase
• Numéro CAS: 9002-04-4
• MDL number: MFCD00008719
• NACRES: NA.25
• EC Index Number: 232-648-7
• UNSPSC Code: 12352204
• Specific activity: 50 NIH units/mg dry wt
• Biological source: bovine plasma

Propriétés

• biological source: bovine plasma
• Quality Segment: 200
• form: solid
• specific activity: 50 NIH units/mg dry wt
• storage temp.: 2-8°C

Description

General description

Thrombin, a glycosylated sodium-activated type II enzyme, is also called coagulation factor IIa. It contains two anion-binding exosites, ABE-I and ABE-II. Thrombin exists as α, β, and γ isoforms at different stages of its generation. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond.

Application

Thrombin has been used in flow cytometry analysis to investigate the basal activation level of circulating platelets in mice. It has also been used to coagulate the plasma and allow the organotypic slice to adhere to the coverslip during culturing.

Biochem/physiol Actions

Thrombin is a serine protease critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. It mediates the conversion of inactive plasma protein fibrinogen (factor I) into biologically active fibrin. Thrombin acts as a pro-coagulant and promotes platelet aggregation. It can also produce anti-coagulant effects. It is used to treat bleeding from capillaries and small venules. During vascular injury, thrombin is crucial for controlling the macrophages, neutrophils, monocytes, SMC, and dendritic cells.

Analysis Note

Appearance (colour): almost white
Appearance (description): powder
Activity (fibrinogen; pH 7.6; 37 °C): ≥ 50 U/mg

Informations sur la sécurité

• signalword: Danger
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Classe de stockage: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• pictograms: GHS08,GHS07
• hcodes: H315,H319,H334,H335