05-363
Anti-Cyclin E Antibody, clone HE12
clone HE12, Upstate®, from mouse
Synonyme(s) :
Anti-CCNE, Anti-pCCNE1
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A propos de cet article
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
HE12, monoclonal
Application:
ICC, IP, WB
Citations:
30
Service technique
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Laissez-nous vous aiderbiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HE12, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CCNE1(898)
General description
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
52 kDa
Immunogen
Recombinant human cyclin E
Application
Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.
Biochem/physiol Actions
human p52cyclin E
Physical form
Format: Purified
Preparation Note
2 years at -20°C or 6 months at 4°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated in immunoblot on human A431 lysate
Other Notes
Replaces: 04-223
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Classe de stockage
12 - Non Combustible Liquids
Que se passe-t-il ?
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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