04-773

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

culture supernatant, clone SA19, from rabbit

• Synonyme(s) : Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: culture supernatant
• Type de produit anticorps: primary antibodies
• Clone: SA19, monoclonal
• Espèces réactives: rat, chicken, mouse, human
• Technique(s): western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_002471.1
• Numéro d'accès UniProt: O14974
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: phosphorylation (pThr850)
• Informations sur le gène: human ... PPP1R12A(4659)

Description

Description générale

Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.

Spécificité

Predicted to cross-react with mouse and rat.

Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.

Immunogène

Epitope: pThr850(Ch) or pThr853(H)

KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Application

Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.

Qualité

Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).

Description de la cible

Doublet at ~130 kDa

Liaison

Replaces: 05-773

Remarque sur l'analyse

Control
HEK293 cell lysate phosphorylated by ROKα/ROCKII.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable