T9650

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, Molecular Biology, non-sterile; 0.2 μm filtered

• Synonym(s): TAE buffer
• NACRES: NA.25
• PubChem Substance ID: 329826908
• UNSPSC Code: 41105319
• MDL number: MFCD00236357

Properties

• grade: Molecular Biology
• Quality Segment: 200
• sterility: non-sterile; 0.2 μm filtered
• product line: BioReagent
• form: solution
• suitability: suitable for gel electrophoresis (after dilution to working concentration)
• foreign activity: Protease, none detected, RNAse, none detected
• SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• InChI key: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Description

Application

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.[1]

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Other Notes

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable