SRP0293

Cathepsin V Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥90% (SDS-PAGE)

• Synonym(s): Cathepsin L2, Cathepsin U
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥436 pmol/min-μg
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in FreeStyle^™ 293-F cells

Properties

• recombinant: expressed in FreeStyle^™ 293-F cells
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• specific activity: ≥436 pmol/min-μg
• mol wt: 37 kDa
• NCBI accession no.: NM_001333.2
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CTSL2(1515)

Description

General description

Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(amino acids 1-16) + cathepsin V (amino acids 18-334), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.

Application

Active human cathepsin V is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin V has been used in a study to assess the cysteine protease attribute of the eukaryotic ribosomal protein S4. Active human cathepsin V has also been used in a study to determine that the simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry.

Physical form

Formulated in 40 mM MES, pH 5.0, 120 mM NaCL and 20% glycerol.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H360D
• pcodes: P201 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable